YAP (D8H1X) XP® Rabbit mAb #14074
产品特性
| 产品货号： | 14074 |
| 规格： | 20 μL; 100 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用对人 YAP 蛋白的羧基末端具有特异性的重组蛋白，对动物进行免疫接种来产生单克隆抗体。表位对应人 YAP 同工型 1 的 Pro435 周围的区域。在人 YAP 蛋白的所有已知同工型中，该序列区域具有 100% 的保守性。 |
| 种属反应性： | 人, 小鼠, 大鼠, 仓鼠 , 猴 |
| 特异性/灵敏度： | YAP (D8H1X) XP® Rabbit mAb 可识别内源水平的总 YAP 蛋白。 |
| 应用： | 蛋白质印迹法 1:1000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫沉淀法 1:50 | |
| IHC Leica BOND 1:100 – 1:400 | |
| 免疫组织化学（石蜡） 1:200 – 1:800 | |
| 免疫荧光法（免疫细胞化学） 1:50 – 1:200 | |
| 流式细胞术（固定/通透） 1:50 – 1:200 | |
| 染色质免疫沉淀法 1:50 | |
| ChIP-seq 1:50 | |
| CUT&RUN 1:50 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097).
The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288).
Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702).
Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288).
The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
产品来源
Cell Signaling Technology