Recombinant Mouse IL-18 Protein (N-6His)
产品特性
| Synonyms: | Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-18; IL-1g; IL1F4; MGC12320 |
| Product Description: | Recombinant Mouse Interleukin-18 is produced by our E.coli expression system and the target gene encoding Asn36-Ser192 is expressed with a 6His tag at the N-terminus. |
| UniProt ID: | P70380 |
| Species: | Mouse |
| Expression System: | E.coli |
| Tag: | N-6His |
| Predicted MW: | 19.7 KDa |
| SDS-PAGE: | 20 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM EDTA, 2mM DTT, 5% Trehaiose, pH 8.0. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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产品背景
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:10653850).
Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532).
Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed:10653850).
Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744).
产品来源
洱畔科技实验室
