Recombinant Mouse CD40L Protein (N-6His)
产品特性
| Synonyms: | CD40 Ligand; CD40LG; HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 |
| Product Description: | Recombinant Mouse CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6×His tag at the N-terminus. |
| UniProt ID: | P27548 |
| Species: | Mouse |
| Expression System: | Human Cells |
| Tag: | N-6His |
| Predicted MW: | 18.8 KDa |
| SDS-PAGE: | 20 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity).
Costimulates T-cell proliferation and cytokine production (By similarity).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity).
Induces the activation of NF-kappa-B (By similarity).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165).
Involved in immunoglobulin class switching (PubMed:1374165).
CD40 ligand, soluble form
Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
产品来源
洱畔科技实验室
