Recombinant Mouse Beta-NGF Protein
产品特性
| Synonyms: | Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB; β-NGF |
| Product Description: | Recombinant Mouse Beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Gly241 is expressed. |
| UniProt ID: | P01139 |
| Species: | Mouse |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 13.5 KDa |
| SDS-PAGE: | 14 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Activity: | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.3-1.5 ng/ml. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:20036257).
Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (PubMed:22649032).
The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse (PubMed:22155786).
In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (PubMed:20036257).
Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (By similarity).
Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer (PubMed:22649032, PubMed:26144237).
The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (PubMed:22649032).
产品来源
洱畔科技实验室
