Recombinant Human VEGF165 Protein
产品特性
| Synonyms: | Vascular Endothelial Growth Factor Isoform 165; VEGF165 |
| Product Description: | Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed. |
| UniProt ID: | P15692-4 |
| Species: | Human |
| Expression System: | Human Cells |
| Tag: | Tag free |
| Predicted MW: | 19.1 KDa |
| SDS-PAGE: | 18-22 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Immobilized Human VEGF165 (Cat#CK-18-2101) at 2μg/ml (100 μl/well) can bind Human VEGF165 antibody. The ED50 of Human VEGF165 antibody is 1.44 ng/ml. |
| Loaded Human VEGFR1-Fc on Protein A Biosensor, can bind Human VEGF165(Cat#CK-18-2101) with an affinity constant of 0.29 nM as determined in BLI assay. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
产品来源
洱畔科技实验室
