Recombinant Human PDGF-BB Protein
产品特性
| Synonyms: | PDGFBB; PDGF-BB |
| Product Description: | Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed. |
| UniProt ID: | P01127 |
| Species: | Human |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 12.42 KDa |
| SDS-PAGE: | 14 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. |
| Endotoxin Level: | Less than 0.02 ng/µg (0.2 EU/µg) as determined by LAL test. |
| Activity: | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
| Purity: | >98% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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产品背景
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395).
Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
产品来源
洱畔科技实验室
