Recombinant Human/Mouse/Rat BMP-2 Protein
产品特性
| Synonyms: | Bone Morphogenetic Protein 2; BMP-2; Bone Morphogenetic Protein 2A; BMP-2A; BMP2; BMP2A |
| Product Description: | Recombinant Human/Mouse/Rat Bone Morphogenetic Protein 2 is produced by our E.coli expression system and the target gene encoding Gln283-Arg396 is expressed. |
| UniProt ID: | P12643 |
| Species: | Human/Mouse/Rat |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 13.3 KDa |
| SDS-PAGE: | 13 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NH4Ac, 4% D-Mannitol, pH 4.0. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:31019025, PubMed:24362451).
Induces cartilage and bone formation (PubMed:3201241).
Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533).
Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754).
In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:20851880, PubMed:16771708).
Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451).
产品来源
洱畔科技实验室
