Recombinant Human IL-2 Protein
产品特性
| Synonyms: | Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2 |
| Product Description: | Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Thr153 is expressed. |
| UniProt ID: | P60568 |
| Species: | Human |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 15.5 KDa |
| SDS-PAGE: | 14 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH 4.0. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Human IL-2 with an affinity constant of 17.0 nM as determined in BLI assay. |
| Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535).
Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed:16293754, PubMed:16477002).
Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed:7973659).
In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:8580378).
This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed:25142963).
Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed:6608729).
Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed:6438535).
Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells
产品来源
洱畔科技实验室
