Recombinant Human IL-17F Protein (C-6His)
产品特性
| Synonyms: | Interleukin-17F; IL-17F; Cytokine ML-1; IL17F |
| Product Description: | Recombinant Human Interleukin-17F is produced by our Mammalian expression system and the target gene encoding Arg31-Gln163 is expressed with a 6His tag at the C-terminus. |
| UniProt ID: | AAH70124.1 |
| Species: | Human |
| Expression System: | Human Cells |
| Tag: | C-6His |
| Predicted MW: | 15.96 KDa |
| SDS-PAGE: | 19 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Activity: | Loaded Biotinylated Human IL-17RA-His-Avi on SA Biosensor, can bind Human IL-17F -His with an affinity constant of 81.3 nM as determined in BLI assay. |
| Immobilized Mouse IL-17RA-Fc at 1μg/ml (100 μl/well) can bind Human IL-17F-His. The ED50 of Human IL-17F-His is 47.94 ng/ml. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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产品背景
Interleukin-17F (IL-17F) exists in a disulfide-linked heterodimer that belongs to the IL-17 family. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL-6, IL-8, and granulocyte colony-stimulating factor. IL-17F can regulate cartilage matrix turnover and stimulates PBMC and T-cell proliferation. IL-17F is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Defects in IL-17F are the cause of familial candidiasis type 6 (CANDF6).
产品来源
洱畔科技实验室
