Recombinant Human FGFB Protein (146AA)
产品特性
| Synonyms: | Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2 |
| Product Description: | Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Pro143-Ser288 is expressed. |
| UniProt ID: | P09038 |
| Species: | Human |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 16.3 KDa |
| SDS-PAGE: | 17 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 0.02% Tween80, pH7.5. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-2.0 ng/ml. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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产品背景
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044).
Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677).
Binds to integrin ITGAV:ITGB3 (PubMed:28302677).
Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677).
Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516).
Can induce angiogenesis (PubMed:23469107, PubMed:28302677).
Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).
产品来源
洱畔科技实验室
