Recombinant Human CD40L Protein
产品特性
| Synonyms: | CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP |
| Product Description: | Recombinant Human CD40 Ligand is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed. |
| UniProt ID: | P29965 |
| Species: | Human |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 16.2 KDa |
| SDS-PAGE: | 15 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| Endotoxin Level: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Activity: | Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L. The ED50 of Human CD40L is 6.05 ng/ml. |
| Loaded Human CD40-His on HIS1K Biosensor, can bind Human CD40L with an affinity constant of 2.43 nM as determined in BLI assay. | |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
产品来源
洱畔科技实验室
