Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145
产品特性
| 产品货号： | 9145 |
| 规格： | 20 μL; 100 μL; 300 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用与小鼠 Stat3 中 Tyr705 周围残基相对应的合成磷酸肽，对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 猴 |
| 特异性/灵敏度： | 仅在 Stat3的酪氨酸 705 位点被磷酸化时，Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 方可检测内源水平的 Stat3 。该抗体不会与磷酸-EGFR 或其他 Stat 蛋白的相应磷酸酪氨酸发生交叉反应。 |
| 应用： | 蛋白质印迹法 1:2000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫沉淀法 1:100 | |
| IHC Leica BOND 1:100 – 1:400 | |
| 免疫组织化学（石蜡） 1:100 – 1:400 | |
| 免疫荧光法（免疫细胞化学） 1:100 – 1:200 | |
| 流式细胞术（固定/通透） 1:100 – 1:400 | |
| 染色质免疫沉淀法 1:100 | |
| ChIP-seq 1:100 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:22306293, PubMed:23084476).
Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214).
May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986).
Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225).
Activated by IL31 through IL31RA (PubMed:15194700).
Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600).
Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214).
Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity).
May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580).
Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476).
Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).
产品来源
Cell Signaling Technology
