Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535
产品特性
| 产品货号： | 2535 |
| 规格： | 20 μL; 100 μL; 300 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用与人 AMPKα2 蛋白的 Thr172 周围残基相对应的合成肽，对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 仓鼠 , 猴, 黑腹果蝇 , 酿酒酵母 |
| 特异性/灵敏度： | Phospho-AMPKα (Thr172) (40H9) Rabbit mAb 仅在苏氨酸 183 磷酸化时才可检测内源性 AMPKα1，而且仅在苏氨酸 172 磷酸化时才可检测内源性 AMPKα2。该抗体不能检测调节性 β 或 γ 亚基。 |
| 应用： | 蛋白质印迹法 1:1000 |
| 免疫沉淀法 1:50 | |
| 免疫组织化学（石蜡） 1:50 – 1:200 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357).
In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357).
AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357).
Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity).
Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed:34077757).
Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity).
AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity).
Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed:11554766, PubMed:11518699, PubMed:15866171, PubMed:17711846, PubMed:18184930).
Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity).
In response to stress, phosphorylates ‘Ser-36’ of histone H2B (H2BS36ph), leading to promote transcription (By similarity).
Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed:14651849, PubMed:18439900, PubMed:20160076, PubMed:21205641).
In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed:21205641).
In that process also activates WDR45/WIPI4 (PubMed:28561066).
Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed:32029622).
In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity).
Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:17486097).
AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity).
May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity).
Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity).
Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed:20074060, PubMed:12519745).
产品来源
Cell Signaling Technology
