NLRP3 (D4D8T) Rabbit mAb #15101
产品特性
| 产品货号： | 15101 |
| 规格： | 100 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用与小鼠 NLRP3 蛋白 Ala306 周围残基对应的合成肽对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠 |
| 特异性/灵敏度： | NLRP3 (D4D8T) Rabbit mAb 可识别 NLRP3 总蛋白的内源水平。 |
| 应用： | 蛋白质印迹法 1:1000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫沉淀法 1:200 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed:19362020, PubMed:23582325, PubMed:26814970, PubMed:27929086, PubMed:26642356, PubMed:27374331, PubMed:28847925, PubMed:28656979, PubMed:30518920, PubMed:36178239).
In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed:19362020, PubMed:16407889, PubMed:18403674, PubMed:26814970, PubMed:26642356, PubMed:27374331, PubMed:28847925).
Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed:16546100, PubMed:17008311, PubMed:26814970, PubMed:26642356, PubMed:27374331, PubMed:28847925).
Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed:22801494).
Under resting conditions, ADP-bound NLRP3 is autoinhibited (By similarity).
NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed:16407888, PubMed:16407890, PubMed:16407889, PubMed:18403674, PubMed:19362020).
Almost all stimuli trigger intracellular K+ efflux (PubMed:23809161).
These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity).
Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed:26814970, PubMed:34615873, PubMed:34861190).
Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed:30487600).
Shows ATPase activity (PubMed:34861190).
产品来源
Cell Signaling Technology