HIF-1α (D1S7W) XP® Rabbit mAb #36169
Specifications
| Product Cat#: | #36169 |
| Product type: | Primary antibody |
| Antigen: | HIF-1α |
| Immunogen: | 使用与人源 HIF-1α 蛋白的 Lys478 周围的残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。 |
| Predicted molecular weight: | 120 kDa |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blotting 1:1000, Immunoprecipitation 1:50, Immunofluorescence (Immunocytochemistry) 1:400 – 1:1600, Flow Cytometry (Fixed/Permeabilized) 1:100 – 1:400, Chromatin IP 1:100, Chromatin IP-seq 1:100, CUT&RUN 1:100 |
| Reactivity: | 人, 小鼠, 猴 |
| Clonality (clone number): | Monoclonal, D1S7W |
| Form: | Liquid |
| Buffer: | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. |
| Concentration: | - mg/mL |
| Purity: | Protein A affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | - |
靶标信息
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:9887100, PubMed:30125331).
Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:9887100, PubMed:30125331).
Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797).
Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5′-TACGTG-3′ within the hypoxia response element (HRE) of target gene promoters (By similarity).
Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:9887100, PubMed:16543236).
Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042).
Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042).
Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298).
产品来源
洱畔科技实验室
