GSK-3β (D5C5Z) XP® Rabbit mAb #12456
产品特性
| Product Cat#: | #12456 |
| Product type: | Primary antibody |
| Antigen: | GSK-3β |
| Immunogen: | 使用人 GSK-3β 蛋白羧基末端特异性重组蛋白对动物进行免疫接种来产生单克隆抗体。 |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blotting 1:1000 |
| Immunoprecipitation 1:50 | |
| Immunohistochemistry (Paraffin) 1:400 – 1:1600 | |
| Immunofluorescence (Immunocytochemistry) 1:200 – 1:800 | |
| Flow Cytometry (Fixed/Permeabilized) 1:100 – 1:400 | |
| Reactivity: | Human, Mouse, Rat, Monkey |
| Clonality (clone number): | Monoclonal, D5C5Z |
| Form: | Liquid |
| Buffer: | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. |
| Concentration: | - mg/ml |
| Purity: | Protein A purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | Glycogen synthase kinase-3 beta; EC:2.7.11.26; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
靶标信息
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 (PubMed:1846781, PubMed:9072970, PubMed:14690523, PubMed:20937854, PubMed:12554650, PubMed:11430833, PubMed:16484495).
Requires primed phosphorylation of the majority of its substrates (PubMed:11430833, PubMed:16484495).
In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed:8397507).
相关文献
1、Chauhan SS, Casillas AL, Vizzerra AD, Liou H, Clements AN, Flores CE, Prevost CT, Kashatus DF, Snider AJ, Snider JM, Warfel NA. PIM1 drives lipid droplet accumulation to promote proliferation and survival in prostate cancer. Oncogene. 2024 Feb;43(6):406-419. doi: 10.1038/s41388-023-02914-0. Epub 2023 Dec 14. PMID: 38097734; PMCID: PMC10837079.
2、Chang Y, Jia HQ, Xu B, Yang L, Xu YT, Zhang JY, Wang MQ, Yang LX, Song ZC. Metadherin inhibits chemosensitivity of triple-negative breast cancer to paclitaxel via activation of AKT/GSK-3β signaling pathway. Chem Biol Drug Des. 2024 Jan;103(1):e14416. doi: 10.1111/cbdd.14416. Epub 2023 Dec 13. PMID: 38093418.
3、Wu X, Wang A, Xu L, Li M, Zhai Q, Wang W, Li C, Jin L. Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells. Cardiovasc Ther. 2023 Dec 13;2023:8817431. doi: 10.1155/2023/8817431. PMID: 38125704; PMCID: PMC10733040.
