靶标信息
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Alexa Fluor® 555-conjugated antibodies absorb light maximally around 555 nm and fluoresce with a peak around 564 nm.Alexa Fluor® 555-conjugated antibodies absorb light maximally around 555 nm and fluoresce with a peak around 565 nm.Alexa Fluor 555 matches the spectra of Cy3 conjugates, so it is suitable for Cy3 filters. The total fluorescence of Alexa Fluor 555 is stronger, and the light stability is better, which provides researchers with a longer image capture time.
