GAPDH (D16H11) XP® Rabbit mAb #5174
产品特性
| 产品货号： | 5174 |
| 规格： | 20 μL; 100 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 通过采用与人 GAPDH 的羧基末端附近的残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 猴 |
| 特异性/灵敏度： | GAPDH (D16H11) XP® Rabbit mAb 可检测 内源性GAPDH 总蛋白水平。 |
| 应用： | 蛋白质印迹法 1:1000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫组织化学（石蜡） 1:800 | |
| 免疫荧光法（免疫细胞化学） 1:200 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794).
Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794).
Modulates the organization and assembly of the cytoskeleton (By similarity).
Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).
Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094).
Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094).
Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501).
Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity).
Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
产品来源
Cell Signaling Technology
