Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (用于生长旺盛型类器官，低生长因子)
产品特性
| Source: | Murine Engelbreth-Holm-Swarm (EHS) tumor |
| Protein Concentration: | 8-12 mg/mL as determined by Lowry assay |
| Endotoxin Level: | ≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay |
| Sterility Testing: | No bacterial or fungal growth detected following 14 days in culture |
| Testing Cell Culture: | Organoid Culture – Cultrex RGF BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells. |
| Gelling Assay – Cultrex RGF BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. | |
| Dome Assay Cultrex RGF BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates. | |
| Tube Formation Assay – Cultrex RGF BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. | |
| Viral Testing: | Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV. |
| Stability: | Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. |
| Shipping Conditions: | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label. |
| Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Species: | Mouse |
产品概述
Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.
Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.