Cultrex Basement Membrane Extract (通用型，用于2D和3D培养，细胞贴壁和侵袭实验等)
产品特性
| Source： | Murine Engelbreth-Holm-Swarm (EHS) tumor |
| Protein Concentration： | 8-12 mg/mL as determined by Lowry assay |
| Endotoxin Level： | ≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay |
| Sterility Testing： | No bacterial or fungal growth detected following 14 days in culture |
| Testing Cell Culture： | Tube Formation Assay – Cultrex BME supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells in a tube formation assay. |
| Viral Testing: | Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV. |
| Stability: | Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. |
| Shipping Conditions: | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label. |
| Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Species: | Mouse |
产品概述
Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.