Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664
产品特性
| 产品货号： | 9664 |
| 规格： | 20 μL; 100 μL; 300 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用与人 caspase-3 Asp175 周围的氨基末端残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 猴 |
| 特异性/灵敏度： | Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 可检测 Asp175 附近裂解活化产生的内源水平的活化 caspase-3 中大片段 (17/19 kDa) 。该抗体无法检测全长 caspase-3 或其他剪切的半胱天冬酶。通过对固定冷冻组织中健康细胞（如胰腺 α 细胞）的特定亚型进行免疫荧光分析可能会观察到非特异性标记。在人和猴样本中可能会观察到细胞浆背景。 |
| 应用： | 蛋白质印迹法 1:1000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫沉淀法 1:50 | |
| 免疫组织化学（石蜡） 1:2000 | |
| 免疫荧光法（免疫细胞化学） 1:400 – 1:1600 | |
| 流式细胞术（固定/通透） 1:6400 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120).
Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375).
At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a ‘216-Asp-|-Gly-217’ bond (PubMed:7774019, PubMed:7596430, PubMed:10497198, PubMed:16374543).
Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity).
Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430).
Involved in the cleavage of huntingtin (PubMed:8696339).
Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690).
Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284).
Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35446120, PubMed:35338844).
Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486).
产品来源
Cell Signaling Technology