Caspase-3 Antibody #9662
产品特性
| 产品货号： | 9662 |
| 规格： | 100 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 多克隆 |
| 免疫原： | 使用与人类 caspase-3 剪切位点周围的残基相对应的合成肽对动物进行免疫接种来产生多克隆抗体。通过蛋白质 A 和肽亲和色谱纯化抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 猴 |
| 特异性/灵敏度： | Caspase-3 Antibody 可检测全长的 caspase-3 (35 kDa) 和剪切产生的 caspase-3 大片段 (17 kDa) 的内源水平。 |
| 应用： | 蛋白质印迹法 1:1000 |
| Simple Western™ 1:10 – 1:50 | |
| 免疫沉淀法 1:50 | |
| 免疫组织化学（石蜡） 1:1000 | |
| 保存： | 保存在 10mM sodium HEPES（pH 为 7.5）、150 mM NaCl、100 µg/ml BSA 和 50% 甘油中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120).
Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:7596430, PubMed:18723680, PubMed:20566630, PubMed:23650375).
At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a ‘216-Asp-|-Gly-217’ bond (PubMed:7774019, PubMed:7596430, PubMed:10497198, PubMed:16374543).
Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity).
Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430).
Involved in the cleavage of huntingtin (PubMed:8696339).
Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690).
Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284).
Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35446120, PubMed:35338844).
Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486).
产品来源
Cell Signaling Technology
