Anti-RFWD2 (Phospho-Ser387) antibody
产品特性
| Product Cat#: | AB-07-2070 |
| Product type: | Primary antibody |
| Antigen: | RFWD2 (Phospho-Ser387) |
| Immunogen: | A synthetic peptide derived from human RFWD2 around the phosphorylation site of serine 385 (T-A-SP-Q-L). The exact amino-acid sequence is proprietary. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2500); ELISA (1:1000) |
| Reactivity: | Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | PBS (without Mg2+ and Ca2+ ), pH 7.4, 0.02% sodium azide and 50% glycerol |
| Concentration: | 1 mg/ml |
| Purity: | Antigen affinity chromatography |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Alternative names: | Constitutive photomorphogenesis protein 1 homolog; COP1; EC 6.3.2.-; HCOP1; RING finger and WD repeat domain protein 2; Ubiquitin- protein ligase COP1 |
靶标信息
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3′-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3′->5′ double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
产品来源
洱畔科技实验室
