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Anti-CRISPR-Cas9 SP antibody

产品货号 产品 品牌 单位 存货 价格 促销价 数量  
AB-06-1161
Anti-CRISPR-Cas9 SP antibody
洱畔/Erpan Tech 有货

产品特性        

Product type: Primary antibody
Antigen: CRISPR-Cas9 SP
Immunogen: Recombinant protein
Species immunized: Rabbit
Isotype: IgG
Applications: Western Blot (1:2000-1:5000); Immunocytochemistry (1:200); Immunohistochemistry (1:50-1:200); Immunofluorescence; Flow Cytometry (1:50-1:100)
Reactivity: Streptococcus pyogenes
Clonality (clone number): Monoclonal (JM11-55)
Form: Liquid
Buffer: Tris-HCl buffer (pH7.4), 1% BSA, 40% glycerol, 0.05% NaN3.
Concentration: 1mg/ml
Purity: Protein A affinity purified
Storage: Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles.
Alternative names: Cas9 antibody
CRISPR-associated endonuclease Cas9/Csn1 antibody
CRISPR-Cas9/Csn1 antibody
csn1 antibody
SpyCas9 antibody
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靶向信息

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795). Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3′-5′ exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820). Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174).

产品来源

洱畔科技实验室

文件下载

Material-Safety-Data-Sheet.pdf (下载47470)