Anti-Caspase-8 Recombinant Rabbit Monoclonal Antibody
产品特性
| Product Cat#: | AB-06-6154 |
| Product type: | Primary antibody |
| Antigen: | Caspase-8 |
| Immunogen: | Synthetic peptide within human Caspase-8 aa 325-374. |
| Molecular weight: | Predicted band size: 55 kDa |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2,000); Immunohistochemistry-Paraffin (1:500-1:1,000); Immunocytochemistry/Immunofluorescence (1:50-1:200); Flow Cytometry (1:500-1:1000); Immunoprecipitation (1-2 μg/sample) |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal, PSH04-77 |
| Form: | Liquid |
| Buffer: | 1*TBS buffer (pH7.4), 40% glycerol, 0.05% BSA, 0.05% NaN3. |
| Concentration: | 1 mg/mL |
| Purity: | Protein A affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | ALPS2B antibody |
| Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody | |
| Apoptotic cysteine protease antibody | |
| Apoptotic protease Mch-5 antibody | |
| Apoptotic protease Mch5 antibody | |
| CAP4 antibody | |
| CASP-8 antibody | |
| CASP8 antibody | |
| CASP8_HUMAN antibody | |
| Caspase8 | |
| Caspase 8 antibody | |
| Caspase 8 apoptosis related cysteine peptidase antibody | |
| Caspase-8 subunit p10 antibody | |
| CED 3 antibody | |
| FADD Like ICE antibody | |
| FADD-homologous ICE/CED-3-like protease antibody | |
| FADD-like ICE antibody | |
| FLICE antibody | |
| FLJ17672 antibody | |
| ICE-like apoptotic protease 5 antibody | |
| MACH alpha 1/2/3 protein antibody | |
| MACH antibody | |
| MACH beta 1/2/3/4 protein antibody | |
| MCH5 antibody | |
| MGC78473 antibody | |
| MORT1 associated ced 3 homolog antibody | |
| MORT1-associated CED-3 homolog antibody | |
| OTTHUMP00000163717 antibody | |
| OTTHUMP00000163720 antibody | |
| OTTHUMP00000163724 antibody | |
| OTTHUMP00000163725 antibody | |
| OTTHUMP00000165062 antibody | |
| OTTHUMP00000165063 antibody | |
| OTTHUMP00000165064 antibody | |
| OTTHUMP00000206552 antibody | |
| OTTHUMP00000206582 antibody |
靶标信息
Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224).
Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224).
Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:16916640, PubMed:8962078, PubMed:9006941).
Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A (PubMed:8681376, PubMed:8681377).
The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224).
The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224).
Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224).
In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at ‘Asp-324’, which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281).
Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (PubMed:32929201, PubMed:34012073).
Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity).
Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity).
May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496).
Cleaves PARP1 and PARP2 (PubMed:8681376).
Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380 (PubMed:18216014, PubMed:27109099).
产品来源
洱畔科技实验室
