铁含量检测试剂盒（比色法） Iron Assay Kit (Colorimetric) (ab83366)

产品概述

Iron Assay Kit ab83366 provides a simple convenient means of measuring ferrous (Fe2+) and/or ferric (Fe3+) iron in biological samples.

Ferric carrier protein will dissociate ferric into solution in the presence of the acid assay buffer. If the assay buffer has a neutral pH, iron will bind tightly to the iron carrier protein. However, under acidic conditions (pH less than 5.5), iron no longer has a binding affinity for the carrier protein and will be dissociated and released into the solution as iron/ferric ions, whose concentration you can measure using this kit. The Iron Assay Buffer in this kit has an acidic pH that enables this release of iron/ferric ions into the solution.

Free ferrous iron (Fe2+) reacts with Iron Probe to produce a stable colored complex with absorbance at 593 nm. Ferric iron (Fe3+) can be reduced to form Fe2+ enabling the measurement of total iron (Fe2+ and Fe3+). The level of ferric iron (Fe3+) is calculated by subtracting ferrous iron from total iron.

A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference.

The kit measures iron in the linear range of 0.4 to 10 nmol or 8 µM to 200 µM.

Iron assay protocol summary:
– add samples and standards to wells
– for Fe2+ assay add assay buffer only, for total iron (Fe2+ and Fe3+) add iron reducer
– incubate for 30 min at 37ºC
– add Iron Probe and incubate for 60 min at 37ºC
– analyze with microplate reader