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β-Actin (13E5) Rabbit mAb #4970

产品货号 产品 品牌 单位 存货 价格 促销价 数量  
4970
β-Actin (13E5) Rabbit mAb #4970
Cell Signaling Technology 有货

产品特性        

产品货号:            4970
规格: 20 μL; 100 μL; 300 μL
物种免疫 :
同种型: IgG
克隆性: 单克隆
免疫原: 使用与人 β-肌动蛋白氨基末端周围的残基相对应的合成肽,对动物进行免疫接种来产生单克隆抗体。
种属反应性: 人, 小鼠, 大鼠, 猴, 牛 , 猪
特异性/灵敏度: β-Actin (13E5) Rabbit mAb 可检测总 β-肌动蛋白的内源水平。尽管细胞质肌动蛋白同工型、β 肌动蛋白和细胞质 γ 肌动蛋白之间有着高序列同源性,但 β-Actin (13E5) Rabbit mAb #4970 不会与细胞质 γ 肌动蛋白或任何其他肌动蛋白同工型发生交叉反应。
应用: 蛋白质印迹法 1:1000
Simple Western™ 1:10 – 1:50
免疫组织化学(石蜡) 1:50 – 1:200
免疫荧光法(冰冻) 1:100 – 1:400
免疫荧光法(免疫细胞化学) 1:100 – 1:400
流式细胞术(固定/通透) 1:100 – 1:400
保存: 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。
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靶标信息

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253).
Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253).
In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

产品来源

Cell Signaling Technology