产品特性

Product type:	Primary antibody
Antigen:	INCENP
Immunogen:	Recombinant protein
Species immunized:	Mouse
Isotype:	IgG1
Applications:	Western Blot (1:800-1:2000); Immunocytochemistry (1:80-1:500); Immunohistochemistry (1:80-1:200); Flow Cytometry (1:80-1:200)
Reactivity:	Human
Clonality (clone number):	Monoclonal(G9-D2)
Form:	Liquid
Buffer:	Tris-HCl buffer (pH7.4), 1% BSA, 40% glycerol, 0.05% NaN3.
Concentration:	1mg/ml
Purity:	Protein A affinity purified
Storage:	Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles.
Alternative names:	binds and activates aurora B and C in vivo and in vitro antibody
	Chromosomal passenger protein antibody
	INCE_HUMAN antibody
	INCENP antibody
	Inner centromere protein antibody
	Inner centromere protein antigens 135/155kDa antibody
	Inner centromere protein antigens 135kD 155kD antibody
	Inner centromere protein INCENP antibody

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靶标信息

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:15316025, PubMed:12925766, PubMed:27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed:27332895). Required for localization of CBX5 to mitotic centromeres (PubMed:21346195). Controls the kinetochore localization of BUB1 (PubMed:16760428).

产品来源

洱畔科技实验室

文件下载

Material-Safety-Data-Sheet.pdf (下载47214)