产品特性
产品货号: | SL100688 |
产品类别: | 细胞转染试剂 |
中文名称: | PolyJet™ 体外DNA转染试剂 |
规格: | 1.0 mL |
存储条件: | 4℃条件可稳定保存12个月。 |
产品货号: | SL100688 |
产品类别: | 细胞转染试剂 |
中文名称: | PolyJet™ 体外DNA转染试剂 |
规格: | 1.0 mL |
存储条件: | 4℃条件可稳定保存12个月。 |
Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. The PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
Features
– Bio-degradable after endocytosis
– Exceptional high titers of virus production
– Equally good for very long DNAs (>89 kb)
– Equally good for both single DNA transfection and multi DNA co-transfection
– High levels of recombinant protein production
– Simple & robust transfection procedure
– Very affordable
Broad Transfection Spectrum for Mammalian Cell Types
Cell Lines |
Efficiency (% GFP) |
Cell Lines |
Efficiency (% GFP) |
McArdle 7777 Hep3D SHEP 3T3-442A COS-7 CV-1 D 407 DHD Pro.b 3LL B16-F10 BAEC BHK-21 Ca Ski CaCo2 CHO HCS-2/8 HEK-293 HeLa HLMEC H-MVEC Huh-7D12 ATT20 SK-N-SH C2C12 HepG2 |
65–70% 67-76% 68-71% 35% 85-90% 60% 70% 70% 80% 85% 51% 80% 88% 60% 88% 61% 86% 88% 72% 59% 72% 46% 29% 46% 72% |
hESCs SN56 MC3T3-E1 Primary melanocyte mESCs L929 MCF-7 MDCK Neuro2A NIH 3T3 PC12 SH-SY5Y SiHa SKOV3 Huh-7 IGROV1 DF-1, Chicken Embryonic Cell 6CSFMEo WEHI 231 A549 LNCap Prim. mouse keratinocyte Prim. human skin fibroblast Prim. human pre-adipocyte Prim. mouse embry. fibroblast |
70% 81% 80% 35% 70% 59% 68% 68% 86% 76% 50% 25% 60% 65% 70% 35% 50% 71% 26% 75% 75 29% 50% 32% 30% |