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PolyJet™ In Vitro DNA Transfection Reagent

产品货号 产品 品牌 单位 存货 价格 促销价 数量  
SL100688
PolyJet™ In Vitro DNA Transfection Reagent
SignaGen 1 mL 有货
1,490.00

产品特性        

产品货号: SL100688
产品类别: 细胞转染试剂
中文名称: PolyJet™ 体外DNA转染试剂
规格: 1.0 mL
存储条件: 4℃条件可稳定保存12个月。
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产品概述

Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. The PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.

Features
– Bio-degradable after endocytosis
– Exceptional high titers of virus production
– Equally good for very long DNAs (>89 kb)
– Equally good for both single DNA transfection and multi DNA co-transfection
– High levels of recombinant protein production
– Simple & robust transfection procedure
– Very affordable

验证数据

Broad Transfection Spectrum for Mammalian Cell Types

Cell Lines

Efficiency (% GFP)

Cell Lines

Efficiency (% GFP)
McArdle 7777
Hep3D
SHEP
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
C2C12
HepG2
6570%
67-76%
68-71%
35%
85-90%
60%
70%
70%
80%
85%
51%
80%
88%
60%
88%
61%
86%
88%
72%
59%
72%
46%
29%
46%
72%
hESCs
SN56
MC3T3-E1
Primary melanocyte
mESCs
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
Huh-7
IGROV1
DF-1, Chicken Embryonic Cell
6CSFMEo
WEHI 231
A549
LNCap
Prim. mouse keratinocyte
Prim. human skin fibroblast
Prim. human pre-adipocyte
Prim. mouse embry. fibroblast
70%
81%
80%
35%
70%
59%
68%
68%
86%
76%
50%
25%
60%
65%
70%
35%
50%
71%
26%
75%
75
29%
50%
32%
30%