β-Catenin (D10A8) XP® Rabbit mAb #8480
产品特性
| 产品货号： | 8480 |
| 规格： | 20 μL; 100 μL |
| 物种免疫 ： | 兔 |
| 同种型： | IgG |
| 克隆性： | 单克隆 |
| 免疫原： | 使用与人 β 连环蛋白 Pro714 周围残基相对应的合成肽，对动物进行免疫接种来产生单克隆抗体。 |
| 种属反应性： | 人, 小鼠, 大鼠, 猴 |
| 特异性/灵敏度： | β-Catenin (D10A8) XP® Rabbit mAb 可检测总 β 连环蛋白的内源水平。 |
| 应用： | 蛋白质印迹法 1:1000 |
| 免疫沉淀法 1:50 | |
| IHC Leica BOND 1:50 – 1:200 | |
| 免疫组织化学（石蜡） 1:50 – 1:200 | |
| 免疫荧光法（冰冻） 1:50 – 1:100 | |
| 免疫荧光法（免疫细胞化学） 1:100 – 1:400 | |
| 流式细胞术（固定/通透） 1:50 – 1:100 | |
| 染色质免疫沉淀法 1:25 | |
| ChIP-seq 1:25 | |
| CUT&RUN 1:25 | |
| 保存： | 保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 µg/ml BSA、50% 甘油和低于 0.02% 的叠氮化钠中。-20℃ 保存。切勿分装抗体。 |
靶标信息
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938).
In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938).
In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938).
Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity).
Acts as a negative regulator of centrosome cohesion (PubMed:18086858).
Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353).
Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423).
Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184).
Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
产品来源
Cell Signaling Technology
