Recombinant Mouse SHH (C25II) Protein
产品特性
| Synonyms: | Sonic Hedgehog Protein; SHH; HHG-1; SHH |
| Product Description: | Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198(Cys25Ile-Ile) is expressed. |
| UniProt ID: | Q62226 |
| Species: | Mouse |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 19.8 KDa |
| SDS-PAGE: | 18-20 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Sonic hedgehog protein
The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed:8824192, PubMed:7891723, PubMed:7736596).
Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed:8824192).
Both activities occur in the reticulum endoplasmic (PubMed:21357747).
Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed:21357747).
Sonic hedgehog protein N-product
The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed:11430830, PubMed:24863049).
Involved in the patterning of the anterior-posterior axis of the developing limb bud (PubMed:15315762).
Essential for axon guidance (PubMed:12679031).
Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity).
In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (By similarity).
产品来源
洱畔科技实验室
