Recombinant Human/Mouse/Rat BDNF Protein
产品特性
| Synonyms: | Brain-Derived Neurotrophic Factor; BDNF; Abrineurin |
| Product Description: | Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed. |
| UniProt ID: | P23560 |
| Species: | Human/Mouse/Rat |
| Expression System: | E.coli |
| Tag: | Tag free |
| Predicted MW: | 13 KDa |
| SDS-PAGE: | 14 KDa, reducing conditions |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| Endotoxin Level: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Activity: | Immobilized Human TrkB-His at 2 ug/ml (100 μl/well) can bind Human BDNF: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF is 47.58 ng/ml. |
| Purity: | >95% |
| Reconstitution: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. |
| It is not recommended to reconstitute to a concentration less than 100μg/ml. | |
| Dissolve the lyophilized protein in distilled water. | |
| Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | |
| Stability and Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. |
| Reconstituted protein solution can be stored at 4-7°C for 2-7 days. | |
| Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
产品背景
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678).
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
BDNF precursor form
Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994).
Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
产品来源
洱畔科技实验室
