Mouse Neutrophil Elastase ELISA kit
Specifications
Product Cat#:
EK-07-1124
Product name:
Mouse Neutrophil Elastase ELISA kit
Target Name:
NE
Species Reactivity:
Mouse
Product Size:
48/96 Tests
Sensitivity:
0.04 ng/ml
Assay range:
0.5-10 ng/ml
Assay Time:
90 minutes
Platform:
Colorimetric Microplate Reader
Conjugate:
HRP
ELISA Type:
Competitive ELISA
Detection Method:
Colorimetric
Storage temperature:
Store at 2-8°C
Stability:
Stable within the expiration date under suggested storage conditions
Shipping condition:
Wet ice
Kit Contents:
Microtiter plate (1x), Enzyme conjugate (1 vial), Standard samples (6 vials),
Substrates (A & B, 2 vials), Stop solution (1 vial), Wash Solution (100x, 1 vial),
Balance solution (1 vial), Instruction (1 copy)
Other Names of Target:
ELANE; HLE; HNE; NE; PMN-E; Neutrophil elastase; Medullasin; Polymorphonuclear leukocyte elastase; Bone marrow serine protease; Human leukocyte elastase; PMN elastase
Target information
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein’s release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
Provider
Erpantech Laboratory
