Mouse Cluster Of Differentiation 73 (CD73) ELISA kit
Specifications
| Product Cat#: | EK-07-0810 |
| Product name: | Mouse Cluster Of Differentiation 73 (CD73) ELISA kit |
| Target Name: | CD73 |
| Species Reactivity: | Mouse |
| Product Size: | 48/96 Tests |
| Sensitivity: | 0.08 ng/ml |
| Assay range: | 2.5-50 ng/ml |
| Assay Time: | 90 minutes |
| Platform: | Colorimetric Microplate Reader |
| Conjugate: | HRP |
| ELISA Type: | Competitive ELISA |
| Detection Method: | Colorimetric |
| Storage temperature: | Store at 2-8°C |
| Stability: | Stable within the expiration date under suggested storage conditions |
| Shipping condition: | Wet ice |
| Kit Contents: | Microtiter plate (1x), Enzyme conjugate (1 vial), Standard samples (6 vials), |
| Substrates (A & B, 2 vials), Stop solution (1 vial), Wash Solution (100x, 1 vial), | |
| Balance solution (1 vial), Instruction (1 copy) | |
| Other Names of Target: | CD73; NT5-E; E5NT; E5-NT; NTE; EN; ENT |
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Target information
CD73 is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
Provider
Erpantech Laboratory
