Human Nuclear factor Kb p65 ELISA kit
Specifications
| Product Cat#: | EK-07-0234 |
| Product name: | Human Nuclear factor Kb p65 ELISA kit |
| Target Name: | NFkB p65 |
| Species Reactivity: | Human |
| Product Size: | 48/96 Tests |
| Sensitivity: | 0.09 µg/mL |
| Assay range: | 0.5-10 ng/mL |
| Assay Time: | 90 minutes |
| Platform: | Colorimetric Microplate Reader |
| Conjugate: | HRP |
| ELISA Type: | Competitive ELISA |
| Detection Method: | Colorimetric |
| Storage temperature: | Store at 2-8°C |
| Stability: | Stable within the expiration date under suggested storage conditions |
| Shipping condition: | Wet ice |
| Kit Contents: | Microtiter plate ( 1x ), Enzyme conjugate ( 1 vial ), Standard samples ( 6 vials ), |
| Substrates ( A & B, 2 vials ), Stop solution ( 1 vial ), Wash Solution ( 100x, 1 vial ), | |
| Balance solution ( 1 vial ), Instruction ( 1 copy ) | |
| Other Names of Target: | NF kB P65; NFKBp65; p65 NF KappaB; p65 NFkB; RELA; Transcription Factor p65; v Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A (nuclear factor of kappa light Polypeptide Gene Enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v Rel Reticuloendotheliosis Viral Oncogene Homolog A (avian); v-rel Reticuloendotheliosis Viral Oncogene homolog A; Avian Reticuloendotheliosis Viral (v rel) Oncogene Homolog A; MGC131774; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells; TF65_HUMAN |
Target information
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells. The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression
Provider
Erpantech Laboratory