Human Cyclophilin A ELISA kit
Specifications
| Product Cat#: | EK-07-0170 |
| Product name: | Human Cyclophilin A ELISA kit |
| Target Name: | CYPA |
| Species Reactivity: | Human |
| Product Size: | 48/96 Tests |
| Sensitivity: | 0.56 ng/mL |
| Assay range: | 5-100 ug/mL |
| Assay Time: | 90 minutes |
| Platform: | Colorimetric Microplate Reader |
| Conjugate: | HRP |
| ELISA Type: | Competitive ELISA |
| Detection Method: | Colorimetric |
| Storage temperature: | Store at 2-8°C |
| Stability: | Stable within the expiration date under suggested storage conditions |
| Shipping condition: | Wet ice |
| Kit Contents: | Microtiter plate ( 1x ), Enzyme conjugate ( 1 vial ), Standard samples ( 6 vials ), |
| Substrates ( A & B, 2 vials ), Stop solution ( 1 vial ), Wash Solution ( 100x, 1 vial ), | |
| Balance solution (1 vial), Instruction (1 copy) | |
| Other Names of Target: | Cyclophilin A; Cyclosporin A Binding Protein; CYPA; CYPH; Peptidyl Prolyl Cis Trans Osomerase A; Peptidyl-prolyl Cis-trans Isomerase A; Peptidylprolyl Isomerase A; PPIA; PPIA Protein; PPIase A; Rotamase A; T Cell Cyclophilin |
Target information
The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes.
Provider
Erpantech Laboratory
