HIF-1 alpha Polyclonal antibody
Specifications
| Product Cat#: | 20960-1-AP |
| Product type: | Primary antibody |
| Antigen: | HIF-1α |
| Immunogen: | HIF-1 alpha fusion protein Ag15198 |
| Predicted molecular weight: | 826 aa, 93 kDa |
| Observed molecular weight: | 120 kDa |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | FC, IF/ICC, IHC, IP, WB, ELISA |
| Reactivity: | Human |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Concentration: | 0.6 mg/mL |
| Purity: | Antigen affinity purification |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | HIF-1 alpha |
Target information
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:9887100, PubMed:30125331).
Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:9887100, PubMed:30125331).
Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797).
Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5′-TACGTG-3′ within the hypoxia response element (HRE) of target gene promoters (By similarity).
Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:9887100, PubMed:16543236).
Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042).
Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042).
Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298).
Provider
Erpantech Laboratory