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Goat anti-rabbit IgG-AP antibody

SKU Product Brand Unit Availability Price Quantity  
AB-06-4775
Goat anti-rabbit IgG-AP antibody
Erpan Tech In stock

Specifications        

Product Cat#: AB-06-4775
Product type: Secondary antibody
Antigen: Rabbit IgG (H+L)
Immunogen: Rabbit IgG (H+L)
Species immunized: Goat
Isotype: IgG
Applications: Western Blot (1:5,000-1:50,000); ELISA (1:5,000-1:50,000)
Reactivity: Goat
Clonality (clone number): Polyclonal
Form: Liquid
Buffer: 1*TBS (pH7.4), 1%BSA, 50%Glycerol.
Concentration: 1 mg/ml
Purity: Protein affinity purified.
Storage: Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles.
Conjugate: AP
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Target information

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.

Provider

Erpantech Laboratory