Anti-TNF-α Rabbit Polyclonal antibody
Specifications
| Product Cat#: | AB-08-0140 |
| Product type: | Primary antibody |
| Antigen: | TNF-α |
| Immunogen: | A synthetic peptide derived from human TNF-α. The exact amino-acid sequence is proprietary. |
| Predicted molecular weight: | 26 kDa |
| Observed molecular weight: | 17 kDa/26 kDa |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2000); Immunohistochemistry-Paraffin (1:500-1:1000); Immunocytochemistry/Immunofluorescence (1:100-1:200) |
| Reactivity: | Human, Mouse, Rat |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH 7.4), 50% Glycerol, 0.05% NaN3. |
| Concentration: | - |
| Purity: | Immunogen affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNF-α |
Target information
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key ‘Ser-418’ residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208).
Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity).
Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).
Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Provider
Erpantech Laboratory
