Anti-TFEB antibody
Specifications
| Product Cat#: | AB-07-2175 |
| Product type: | Primary antibody |
| Antigen: | TFEB |
| Immunogen: | The antiserum was produced against synthesized peptide derived from N-terminal of human TFEB. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2500); Immunohistochemistry (1:50-1:100); ELISA (1:15000) |
| Reactivity: | Human, Mouse |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration: | 1 mg/ml |
| Purity: | Antigen affinity chromatography |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Alternative names: | Transcription factor EB, TFEB |
Target information
Transcription factor that specifically recognizes and binds E-box sequences (5′-CANNTG-3′). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5′-GTCACGTGAC-3′) present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).
Provider
Erpantech Laboratory
