Anti-sfGFP antibdody
Specifications
| Product Cat#: | AB-06-0311 |
| Product type: | Primary antibody |
| Antigen: | sfGFP |
| Immunogen: | Synthetic peptide RDHMVLHEYVNAAGITC linked to KLH |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:1000) |
| Reactivity: | Mouse, Rat and Human |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 1% BSA, 40% Glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/ml |
| Purity: | Antigen affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: |
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Target information
Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.
Provider
Erpantech Laboratory
