Anti-rabbit IgG for IP Nano-secondary antibody (HRP)

Target information

Anti-Rabbit IgG Nano-secondary antibody for IP (HRP) is based on Monovalent, recombinant single domain antibodies to Rabbit IgG coupled to HRP, and the Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of Rabbit IgG selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, the denatured Rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-Rabbit IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of Rabbit IgG, no reactivity with the reduced, SDS-denatured forms. When a protien was immunoprecipitated by an antibody derived from Rabbit, you can still use primary antibody derived from Rabbit to detect this protien. Using Anti-Rabbit IgG for IP Nano-secondary antibody (HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.