Anti-mouse IgG for IP Nano-secondary antibody (HRP)
Specifications
| Product Cat#: | AB-06-4812 |
| Product type: | Primary antibody |
| Antigen: | Mouse IgG |
| Immunogen: | Mouse IgG |
| Species immunized: | Alpaca |
| Applications: | Western Blot (1:1000-1:10000) |
| Reactivity: | Mouse |
| Clonality (clone number): | Monoclonal |
| Form: | Liquid |
| Buffer: | 1*PBS (pH7.4), 10 mg/ml BSA, 50% glycerol and 100 mM trehalose. |
| Concentration: | 1 mg/ml |
| Purity: | Immunogen affinity purified. |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | HRP |
Target information
Anti-mouse IgG Nano-secondary antibody for IP (HRP) is based on Monovalent, recombinant single domain antibodies to mouse IgG coupled to HRP, and the Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protien was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protien. Using Anti-mouse IgG for IP Nano-secondary antibody (HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.
Provider
Erpantech Laboratory
