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Anti-MHC Class II antibody

SKU Product Brand Unit Availability Price Quantity  
AB-06-2881
Anti-MHC Class II antibody
Erpan Tech In stock

Specifications        

Product Cat#: AB-06-2881
Product type: Primary antibody
Antigen: MHC Class II
Immunogen: Recombinant protein
Species immunized: Rabbit
Isotype: IgG
Applications: Western Blot (1:500-1:1000); Immunoprecipitation (1:10-1:50); Immunohistochemistry (1:50-1:200); Immunocytochemistry (1:50-1:100); Immunofluorescence
Reactivity: Human
Clonality (clone number): Monoclonal (JA10-94)
Form: Liquid
Buffer: Tris-HCl buffer (pH7.4), 1% BSA, 40% Glycerol, 0.05% NaN3.
Concentration: 1 mg/ml
Purity: Protein A affinity purified
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles
Alternative names: D6S221E antibody
DMA antibody
DMB antibody
DP beta 1 antibody
DP beta 1 chain antibody
DP(W4) beta chain antibody
DPB 1 antibody
DPB1 antibody
DPB1_HUMAN antibody
DRB antibody
H2Ea antibody
HLA class II histocompatibility antigen antibody
HLA class II histocompatibility antigen DM beta chain antibody
HLA class II histocompatibility antigen, DP beta 1 chain antibody
HLA class II histocompatibility antigen, DP(W4) beta chain antibody
HLA DMB antibody
HLA DP1A antibody
HLA DPB1 antibody
HLA SB alpha chain antibody
HLA-A antibody
HLA-A histocompatibility type antibody
HLA-DP antibody
HLA-DP histocompatibility type, beta-1 subunit antibody
HLA-DP1B antibody
HLA-DPB antibody
HLA-DPB1 antibody
HLADM antibody
HLADP1B antibody
HLASB antibody
HLASB histocompatibility type antibody
Human MHC class II HLA SB alpha antibody
LA class II histocompatibility antigen DP alpha 1 chain antibody
Major histocompatibility complex class II antibody
Major histocompatibility complex class II DP alpha 1 antibody
Major histocompatibility complex class II DP beta 1 antibody
Major histocompatibility complex, class I, A antibody
MHC class II antigen DMB antibody
MHC class II antigen DPB1 antibody
MHC class II DP3 alpha antibody
MHC class II DPA1 antibody
MHC class II HLA-DP-beta-1 antibody
MHC DPB1 antibody
MHC HLA DPB1 antibody
PLT1 antibody
Primed lymphocyte test 1 antibody
RING6 antibody
RING7 antibody

 

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Target information

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Provider

Erpantech Laboratory

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MSDS-AB-06-2881.pdf (101 downloads )