Anti-LC3B antibody (KO Validated)
Specifications
| Product Cat#: | AB-08-0122 |
| Product type: | Primary antibody |
| Antigen: | LC3B |
| Immunogen: | A synthetic peptide of human LC3B. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:400-1:2000); Immunohistochemistry (1:100-200); Immunoprecipitation (1:100-1:200); Immunofluorescence (1:100-1:200) |
| Reactivity: | Human, Rat, Mouse |
| Clonality (clone number): | Monoclonal |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH7.4), 50% Glycerol, 0.05% BSA, 0.02% NaN3. |
| Purity: | Protein A affinity purification. |
| Storage: | Aliquot and freeze at -20°C. Avoid multiple freeze/thaw cycles. |
| Conjugate: | Unconjugated |
| Alternative names: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B; LC3A/LC3B |
Target information
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329).
Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329).
In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758).
While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329).
Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205).
Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).
Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY’s actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355).
Provider
Erpantech Laboratory