Anti-INCENP Antibody
Specifications
| Product Cat#: | AB-06-2451 |
| Product type: | Primary antibody |
| Antigen: | INCENP |
| Immunogen: | Recombinant protein |
| Species immunized: | Mouse |
| Isotype: | IgG1 |
| Applications: | Western Blot (1:800-1:2000); Immunocytochemistry (1:80-1:500); Immunohistochemistry (1:80-1:200); Flow Cytometry (1:80-1:200) |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal(G9-D2) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH 7.4), 1% BSA, 40% glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | binds and activates aurora B and C in vivo and in vitro antibody |
| Chromosomal passenger protein antibody | |
| INCE_HUMAN antibody | |
| INCENP antibody | |
| Inner centromere protein antibody | |
| Inner centromere protein antigens 135/155kDa antibody | |
| Inner centromere protein antigens 135kD 155kD antibody | |
| Inner centromere protein INCENP antibody |
Target information
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:15316025, PubMed:12925766, PubMed:27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed:27332895). Required for localization of CBX5 to mitotic centromeres (PubMed:21346195). Controls the kinetochore localization of BUB1 (PubMed:16760428).
Provider
Erpantech Laboratory