Anti-HLA E antibody
Specifications
| Product Cat#: | AB-06-2208 |
| Product type: | Primary antibody |
| Antigen: | HLA E |
| Immunogen: | Recombinant HLA-E of human origin |
| Species immunized: | Mouse |
| Isotype: | IgG1 |
| Applications: | Western Blot (1:150-1:1000); Immunoprecipitation (1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)); Flow Cytometry( 1 μg per 1 x 106 cells)) |
| Reactivity: | Human |
| Clonality (clone number): | Monoclonal (4G23) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | HLA class I histocompatibility antigen E alpha chain antibody |
| EA1.2 antibody | |
| EA2.1 antibody | |
| HLA 6.2 antibody | |
| HLA class I histocompatibility antigen alpha chain E antibody |
Target information
Non-classical major histocompatibility class Ib molecule involved in immune self-nonself discrimination. In complex with B2M/beta-2-microglobulin binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules (VL9 peptides) (PubMed:9754572, PubMed:18083576, PubMed:18339401). Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self (PubMed:9754572, PubMed:9486650, PubMed:17179229, PubMed:18083576). Upon cellular stress, preferentially binds signal sequence-derived peptides from stress-induced chaperones and is no longer recognized by NK cell inhibitory receptor KLRD1-KLRC1, resulting in impaired protection from NK cells (PubMed:12461076). Binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules and acts as a ligand for NK cell activating receptor KLRD1-KLRC2, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal-fetal tolerance during pregnancy (PubMed:9754572, PubMed:30134159). Besides self-peptides, can also bind and present pathogen-derived peptides conformationally similar to VL9 peptides to alpha-beta T cell receptor (TCR) on unconventional CD8+ cytotoxic T cells, ultimately triggering antimicrobial immune response (PubMed:16474394, PubMed:30087334).
Provider
Erpantech Laboratory
