Anti-Exportin-5 antibody
Specifications
| Product cat: | AB-06-1625 |
| Product type: | Primary antibody |
| Antigen: | Exportin-5 |
| Immunogen: | Recombinant protein within human Exportin-5 aa 1-200. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:900-1:2400); Immunohistochemistry (1:90-1:260); Immunocytochemistry (1:100-1:250); Flow Cytometry (1:70-1:260); Immunofluorescence |
| Reactivity: | Mouse, Rat and Human |
| Clonality (clone number): | Monoclonal (JE40-91) |
| Form: | Liquid |
| Buffer: | Tris-HCl buffer (pH7.4), 1% BSA, 40% glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | Exp 5 antibody |
| Exp5 antibody | |
| Exportin 5 antibody | |
| Exportin-5 antibody | |
| Exportin5 antibody | |
| FLJ14239 antibody | |
| FLJ32057 antibody | |
| FLJ45606 antibody | |
| KIAA1291 antibody | |
| OTTHUMP00000016472 antibody | |
| Ran binding protein 21 antibody | |
| Ran-binding protein 21 antibody | |
| Ranbp 21 antibody | |
| Ranbp21 antibody | |
| Xpo 5 antibody | |
| xpo5 antibody | |
| XPO5_HUMAN antibody |
Target information
Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.
Mediates the nuclear export of micro-RNA precursors, which form short hairpins (PubMed:14681208, PubMed:14631048, PubMed:15613540). Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5′-overhang in a sequence-independent manner, have only a short 3′-overhang, and that have a double-stranded length of at least 15 base-pairs (PubMed:19965479). Binding is dependent on Ran-GTP (PubMed:19965479).
(Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
Provider
Erpantech Laboratory