Anti-EIF2S1 Rabbit Polyclonal antibody
Specifications
| Product Cat#: | AB-06-4957 |
| Product type: | Primary antibody |
| Antigen: | EIF2S1 |
| Immunogen: | Recombinant protein within human EIF2S1 aa 116-315/315. |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2000); Immunohistochemistry-Paraffin (1:500-2000); Immunofluorescence-Cell (1:100-1:200); Flow Cytometry (1:500-1:1000) |
| Reactivity: | Human, Mouse, Rat |
| Clonality (clone number): | Polyclonal |
| Form: | Liquid |
| Buffer: | 1*PBS buffer (pH 7.4), 0.1% BSA, 40% glycerol, 0.05% NaN3. |
| Concentration: | 1 mg/mL |
| Purity: | Immunogen affinity purified. |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | EIF 2 alpha antibody |
| EIF 2 antibody | |
| EIF 2A antibody | |
| EIF 2alpha antibody | |
| eIF-2-alpha antibody | |
| eIF-2A antibody | |
| EIF-2alpha antibody | |
| EIF2 alpha antibody | |
| EIF2 antibody | |
| EIF2A antibody | |
| EIF2S1 antibody | |
| Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody | |
| Eukaryotic translation initiation factor 2 subunit 1 alpha antibody | |
| Eukaryotic translation initiation factor 2 subunit 1 antibody | |
| Eukaryotic translation initiation factor 2 subunit alpha antibody | |
| IF2A_HUMAN antibody |
Target information
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705).
This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:16289705).
Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PubMed:16289705).
In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PubMed:16289705).
EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352).
Provider
Erpantech Laboratory