Anti-DGCR8 antibody
Specifications
| Product Cat#: | AB-06-1368 |
| Product type: | Primary antibody |
| Antigen: | DGCR8 |
| Immunogen: | Recombinant protein |
| Species immunized: | Rabbit |
| Isotype: | IgG |
| Applications: | Western Blot (1:500-1:2000); Immunocytochemistry (1:100-1:200); Immunofluorescence; Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Clonality (clone number): | Monoclonal (ST04-79) |
| Form: | Liquid |
| Buffer: | 1*TBS (pH 7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/ml |
| Purity: | Protein A affinity purified |
| Storage: | Aliquot and freeze at -20℃. Avoid multiple freeze/thaw cycles. |
| Alternative names: | DGCRK6 antibody |
| C22orf12 antibody | |
| D16H22S788E antibody | |
| D16Wis2 antibody | |
| DGCR 8 antibody | |
| Dgcr8 antibody | |
| DGCR8 microprocessor complex subunit antibody | |
| DGCR8_HUMAN antibody | |
| DGCRK 6 antibody | |
| DiGeorge syndrome critical region 8 antibody | |
| DiGeorge syndrome critical region gene 8 antibody | |
| Gy1 antibody | |
| Microprocessor complex subunit DGCR8 antibody | |
| Pasha antibody |
Target information
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal (By similarity).
Provider
Erpantech Laboratory
